Journal: iScience

Article Title: The different impact of drug-resistant Leishmania on the transcription programs activated in neutrophils

doi: 10.1016/j.isci.2024.109773

Figure Lengend Snippet:

Article Snippet: NovaSeq 6000 S1 Reagent Kit v1.5 (100 cycles) , Illumina , Cat#20028319.

Techniques: Control, Recombinant, Isolation, Selection, Staining, Reverse Transcription, Purification, SYBR Green Assay, Picogreen Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Nature Communications

Article Title: GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling

doi: 10.1038/s41467-025-67967-z

Figure Lengend Snippet: A GPR4 regulates JAK2 and STAT3 phosphorylation via western blotting. n = 3. Scale bar: 100 µm. B Representative IF images of STAT3-Y705 phosphorylation regulated by GPR4. C Quantitative analysis of STAT3-Y705 phosphorylation in GPR4 - OE SW480 cell line and ( D ) GPR4 - KD HCT116 cell lline. Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. E Static treatment reduces both pSTAT3-Y705 and the expression of LOXL2, COL1A1, and TGF-β in GPR4 - OE SW480 cells. F RT-PCR analysis for LOXL2 in SW480 cell lines after receiving Stattic treatment. Two-tailed t test, n = 3 biologically independent samples. G RT-PCR analysis for TGF-β in SW480 cell lines after receiving Stattic treatment. Data are presented as mean ± SD.Two-tailed t test, n = 3 biologically independent samples. H STAT3 transcription factor binding site. I In SW480 cells, STAT3 binds to the LOXL2 promoter region confirmed via ChIP assays. J STAT3 binds to the TGF-β promoter region confirmed via ChIP assays. K RT-PCR assesses the transcriptional activity of LOXL2 promoter regions and TGF-β promoter region L . Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. ns > 0.05.

Article Snippet: Subsequently, Transcription Factor Staining Kit (Elabscience, E-CK-A108) was applied for cells fixation and Permeabilization as recommended method.

Techniques: Phospho-proteomics, Western Blot, Two Tailed Test, Expressing, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Activity Assay

Journal: Oncology Reports

Article Title: HSH2D contributes to methotrexate resistance in human T-cell acute lymphoblastic leukaemia

doi: 10.3892/or.2020.7772

Figure Lengend Snippet: Effect of MTX on the biological function of HUT-78 cells. (A) Chemical formula of MTX. (B) Effect of different concentrations of MTX on cell survival. (C) Flow cytometric analysis of (D) the effects of different concentrations of MTX on cells effects of apoptosis. Data are presented as the mean ± SD, n=3, and were analysed using a one-way ANOVA with Tukey's test. *P<0.05, **P<0.01, ***P<0.005 vs. NC. MTX, methotrexate; NC, negative control.

Article Snippet: Cell apoptosis was evaluated using Annexin V FITC/PI apoptosis detection kit (Beyotime Institute of Biotechnology; cat. no. C1062).

Techniques: Negative Control

Journal: Oncology Reports

Article Title: HSH2D contributes to methotrexate resistance in human T-cell acute lymphoblastic leukaemia

doi: 10.3892/or.2020.7772

Figure Lengend Snippet: HSH2D is essential for MTX resistance of HuT-78 cells. (A) Silencing efficacy was evaluated using reverse transcription-quantitative PCR after transfection of two siRNAs and a NC of HSH2D. (B) Knockdown of HSH2D promoted apoptosis induced by MTX treatment in both MTX-resistant cell lines. (C) IC 50 value of MTX was detected for both sensitive and resistant cells using a Cell Counting Kit-8 assay. (D) EdU results of the cell proliferation of drug resistant strains with HSH2D knockdown. (E) Flow cytometry was used to detected (F) the cell cycle in drug resistant strains with HSH2D knockdown. Data are presented as the mean ± SD, n=3, and were analysed using a two-tailed Student's t-test or one-way ANOVA. *P<0.05 and **P<0.01 vs. siNC, siRNA negative control; MTX, methotrexate; HSH2D, haematopoietic SH2 domain containing.

Article Snippet: Cell apoptosis was evaluated using Annexin V FITC/PI apoptosis detection kit (Beyotime Institute of Biotechnology; cat. no. C1062).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Knockdown, Cell Counting, Flow Cytometry, Two Tailed Test, Negative Control